Method of Test for Veterinary Drug Residues in Foods -
Fast Extraction Method for Multiresidue Analysis of β-Agonists

FaPEx-VAG is a high-efficiency and operate easily,Fast Extraction Method for Multiresidue Analysis of β-Agonists can improve the efficiency of inspection and check the food safety for people!

FaPEx-VAG is suitable for  Analysis of β-Agonists, and can be applied to 21 pharmaceutical tests of  Analysis of β-Agonists method(TFDA Download)

Announcement method		 Number of medicines The actual amount
Method of Test for Veterinary Drug Residues in Foods - Fast Extraction Method for Multiresidue Analysis of β-Agonists 21 21
 

Fast Extraction Method for Multiresidue Analysis of β-Agonists

Hydrolysis and extraction

1.Prepare 2g 
2.Add internal solution 及0.2 M Sodium acetate buffer solution15mL 
3.Add  homogenizer 、Mix by Vortex for 10 minutes 
4.Add β-glucuronidase 100 uL 
5.37℃ Water bath for 1 hour
6.Add hydrochloric acid 2 mL ,then vortex for 10 minutes
7.4℃ 10000 rpm centrifuge for 10 minutes
8. Prepare supernatant with 4℃ 5000 rpm ,then centrifuge for 10 minutes

 

Pre-processing time: 300 minutes (20 samples)

Purification

1Solid Phase Extraction
2.Dry with Nitrogen at 65°C 
3.With 5 mM ammonium acetate：Methanol(9:1, v/v) 1 mL，Vortex to mix and dissolve
4.Centrifuge for 5 minutes(5000 ×g )，Take the supernatant as the sample solution 
5Take 500 μL of stock solution and add 5 mM ammonium acetate: Methanol (9:1, v/v) solution to make the volume 1000 μL
FaPEx-VAG Operation Procedure
Pre-processing time: 60 minutes (20 samples)

FaPEx-VAG Operation process video

 FaPEx performance recognized by​ 2018 FAPAS 02349
  Recruitment(µg/kg) Measured value(µg/kg) Z-score Evaluation
Clenbuterol 3.88 3.87 0.0 Acceptable
Clenpenterol 3.14 2.82 -0.5 Acceptable
Ractopamine 3.89 3.43 -0.5 Acceptable

•Pesticide substance：β-agonist  •Sample  Matrix：Beef liver
 

FaPEx-VAG FQA

 What are differences between FaPEx-VAG and FaVEx-AG?Can FaVEx-AG be exchanged?

FaPEx-VAG and FaVEx-AG are exactly the same products, and the formula and process are exactly the same. If customers have already purchased FaVEx-AG, GETECH can issue a certificate confirming that the two products are completely consistent and don't need to exchange it.

 Why are there only 7 internal standards for the suggested method?

The original  β-Agonistsannouncement method requires 18 internal standards, which imposes a large cost burden on the laboratory. Therefore, when developing the method, a total of 7 suitable internal standards were selected according to factors such as the chemical properties of the drug and the peak time. The second validation experiment is equally representative, and these data have been sent to TFDA for review.