FAST PESTICIDE EXTRACTION
 
 
What is FaPEx？

FaPEx is the abbreviation of Fast Pesticide Extraction, which is the fast extraction technique of pesticide residue in agricultural products. FaPEx is now the fastest method in the world. This technique was researched and developed by Taiwan Agricultural Chemicals and Toxic Substances Research Institute (TACTRI), and was awarded a patent by the Taiwanese government in 2015.

FaPEx is easier and more efficient than the QuEChERS method. FaPEx provides the following benefits: triple the total efficiency, use less operating equipment, cut at least 60% of labor costs, use at least 50% less organic solvents, and have less waste.

FaPEx Benefits

1. Extract more than 300 pesticides simultaneously.

2. Easier and more efficient extraction.

3. Cut at least 60% of labor costs.

4. Less operating equipment.

5. Less organic solvent and waste.


General Vegetable and Fruit samples


Chlorophyll samples


Cereal samples


Tea samples
The Process of FaPEx and QuEChERS
  FaPEx Procedure QuEChERS Procedure
Sample
preparation
and
extraction
  1. Add 5ml volume of 1% Acetic Acid(HOAc) in Acetonitrile(MeCN) solution.
  2. Mix by Vortex 3. Transfer the combined test solution into the 10mL tube.
 1.Add 10ml volume of 1% Acetic Acid(HOAc) in Acetonitrile(MeCN) solution.
2. Add homogenizer.
3. Add extraction powder.
4. Vortex vigorously.
Sample
extract
cleanup		 Clean and Extract with FaPEx kit 5. Centrifugation.
6 .Collect supernatants.
7. Add clean-up powder.
8. Vortex, centrifuge.
9. Add up to volume.

FaPEx Operation Procedure
FaPEx-gen and FaPEx-chl
Weigh the homogenized sample to 2g+0.004g
FaPEx-cer and FaPEx-tea
Weigh the homogenized sample to 1g+0.002g
Add 2mL pure water, stand for 10 minutes
 
Add 10 mL volume of 1% Acetic Acid(HOAc) in Acetonitrile(MeCN) solution, then vortex for 30 seconds
Transfer 5 mL test solution into FaPEx kit, controlling the liquid flow reat a 1 drop per second
Collect all the test solution
 
Prepare 1 mL
LC-MS/MS Analysis
Dry 1 mL with Nitrogen
Add an Acetone : Hexane (1:1,v/v) solution into test solution to 1mL
GC-MS/MS Analysis
FaPEx performance recognized by FAPAS 19195QC
FaPEx participated in the 2015 FAPAS 19195 ability test through the Drug Toxicology Laboratory of the Council of Agriculture in 2015,
and the performance evaluation results were acceptable.
Matrix: Lettuce FaPEx FAPAS
Pesticide substance Instrument  Residue (ug/g)  Recovery (%) Evaluation
Isoproturon GCMSMS 0.143 98% Acceptable
Pirimiphos-methyl GCMSMS 0.061 110% Acceptable
alpha-Endosulfan GCMSMS 0.115 93% Acceptable
Endosulfan sulfate GCMSMS 0.110 103% Acceptable
Carbaryl LCMSMS 0.156 100% Acceptable
Dimethomorph LCMSMS 0.030 98% Acceptable
Indoxacarb LCMSMS 0.077 95% Acceptable
Trifloxystrobin LCMSMS 0.081 99% Acceptable
FaPEx test results by major 14 labs in Taiwan
Over 98% of pesticide residues for 4 matrixes have a recovery rate between 60%~140%.
Over 93% of pesticide residues for 4 matrixes have an RSD of less than 20%.
 
Matrix Bok Choy Tomato Rice Green Tea
R<60 5 3 4 5
60 308 310 309 307
R>140 0 0 0 1
 
RSD<20 302 303 303 291
RSD>20 11 10 10 22
FaPEx Product Information
Part Number Product Description Q'ty
FaPEX-GEN50 Fast Pesticide Extraction Kit- General sample 50/pk
FaPEX-CHL50 Fast Pesticide Extraction Kit- Chlorophyll sample 50/pk
FaPEX-CER50 Fast Pesticide Extraction Kit- Cereal sample 50/pk
FaPEX-TEA50 Fast Pesticide Extraction Kit- Tea sample 50/pk